ABSTRACT
Objective:To analyze the expression and significance of interleukin-17 (IL-17), IL-23 and IL-22 in Klebsiella pneumoniae (KPN) pneumonia in rats. Methods:A total of 40 male SPF rats were randomly divided into the control group and the experimental group according to the simple number random table method, with 20 rats in each group. The control group was simultaneously dropped with 2 mL normal saline, while the experimental group was inoculated with a 2 mL suspension of 0.9×10 9 CFU/mL. The lung tissue was taken for pathological and bacteriological examination. Arterial partial pressure of oxygen (PaO 2), serum IL-17, IL-23 and IL-22 levels were detected after surgery for 4 h, 1 d, 3 d and 5 d. White blood cell count (WBC) and absolute neutrophil count (ANC) were performed in bronchoalveolar lavage fluid (BALF), and the relevance between IL-17, IL-23, IL-22 and PaO 2, and WBC, ANC in BALF was analyzed. Results:After surgery for 4 h, 1 d, 3 d and 5 d, the CFU count of lung tissue in experimental group rats decreased over time. PaO 2 in experimental group was significantly lower than that in the control group after surgery for 4 h, 1 d, 3 d and 5 d ( P<0.05), while WBC and ANC in BALF in the experimental group were significantly higher at the same time ( P<0.05). The levels of IL-17 and IL-23 in serum in the experimental groups were significantly higher than those in the control group after surgery for 4 h, 1 d, 3 d and 5 d ( P<0.05), and the levels of IL-22 in serum in the experimental groups were significantly lower than those in the control group at the same time ( P<0.05). Pearson analysis showed that IL-17 and IL-23 were negatively correlated with PaO 2 ( P<0.05) and positively correlated with WBC and ANC ( P<0.05). IL-22 was positively correlated with PaO 2 ( P<0.05), and negatively correlated with WBC and ANC ( P<0.05). Conclusions:Serum IL-17, IL-23, and IL-22 levels were significantly altered during the course of KPN rats, and serum IL-17, IL-23, and IL-22 levels were correlated with severity of illness.
ABSTRACT
Objective:To investigate the clinical effect of CT angiography combined with interventional therapy in the treatment of diabetic foot.Methods:A total of 80 patients with diabetic foot who were treated at the Central Hospital of Shandong Xinwen Mining Group Co., Ltd.from December 2017 to June 2019 were selected in the study.The patients were divided into two groups according to random double-blind method, with 40 cases in each group.The control group received open surgery, and the observation group received CT angiography combined with interventional surgery.The oxidative stress reactions[superoxide dismutase (SOD), nitric oxide (NO), lipid peroxide (MDA), nitric oxide synthase activity (iNOS)]before and after surgery, and postoperative complications were compared between the two groups.Results:The success rate of surgical treatment in the observation group was 100.0%, which in the control group was 95.0%, the difference was not statistically significant(χ 2=0.513, P=0.474). The operation time[(28.49±3.64)min], intraoperative blood loss[(2.13±0.47)mL], limb activity time[(1.24±0.51)d], hospital stay[(2.17±0.24)d]in the observation group were lower than those in the control group[(52.47±5.18)min, (57.42±6.87)mL, (2.86±0.63)d, (6.45±0.52)d], the differences were statistically significant( t=23.955, 50.782, 25.349, 47.265, all P<0.05). The incidence of postoperative complications in the observation group was 5.00%, which was lower than that in the control group(27.50%), the difference was statistically significant(χ 2=5.878, P=0.015). At postoperative 7d, the levels of SOD, NO, iNOS and MDA in the observation group were (354.26±21.83)U/L, (41.95±6.43)μmol/L, (77.12±1.86)U/L, (11.68±2.59)nmol/L, respectively, which in the control group were (355.11±32.78)U/L, (42.05±8.42)μmol/L, (78.22±1.85)U/L, (11.55±2.45)nmol/L, respectively, the differences were no statistically significant between the two groups (all P>0.05). Conclusion:CT angiography combined with interventional therapy in the treatment of patients with diabetic foot has high success rate of surgery, which can reduce the incidence of postoperative complications, and promote recovery of patients after surgery.However, CT angiography combined with intervention will increase the oxidative stress response of patients after surgery.Therefore, anti-oxidative stress drugs should be used to improve the prognosis of patients and improve the effect of interventional therapy.